pet30a expression vector (New England Biolabs)
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pet30a expression vector
Pet30a Expression Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+expression+vector/NdeI/pmc11125983-59-46-53
Average 99 stars, based on 4837 article reviews
Pet30a Expression Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+expression+vector/NdeI/pmc11125983-59-46-53
Average 99 stars, based on 4837 article reviews
pet30a expression vector - by Bioz Stars,
2026-09
99/100 stars
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Amplification:Article Title: Development of a Gold Nanoparticle Vaccine against Enterohemorrhagic Escherichia coli O157:H7 Article Snippet: EHEC (EDL933) DNA was isolated via the use of a Qiagen DNeasy blood and tissue kit, following the manufacturer’s directions. .. Sequences encoding LomW (GenBank protein accession no. 12514345) and EscC (GenBank protein accession no. 12518466) were amplified via the use of Phusion polymerase (New England BioLabs) and cloned into a Article Title: Development of a Gold Nanoparticle Vaccine against Enterohemorrhagic Escherichia coli O157:H7 Article Snippet: EHEC (EDL933) DNA was isolated via the use of a Qiagen DNeasy blood and tissue kit, following the manufacturer’s directions. .. Sequences encoding LomW (GenBank protein accession no. 12514345 ) and EscC (GenBank protein accession no. 12518466 ) were amplified via the use of Phusion polymerase (New England BioLabs) and cloned into a pET30a( Article Title: Use of Reverse Vaccinology in the Design and Construction of Nanoglycoconjugate Vaccines against Burkholderia pseudomallei Article Snippet: 321 322 Cloning and Expression of Burkholderia Antigens 323 B. mallei ATCC 23344 DNA was isolated via QIAGEN® DNeasy® Blood and Tissue Kit, 324 according to manufacturer’s directions. .. Sequences encoding OmpW (BMA2010/ BPSL2704), 325 Porin OpcP1 (BMAA1122/BPSS0708), Hemagglutinin (BMAA1324/BPSS0908), Hcp1 326 (BMAA0742/BPSS1498), FlgD (BMA3327/BPSL0272), OpcP porin (BMAA1353/ BPSS0879), 327 porin (BMAA0599/BPSS0757) and FlgL (BMA3336/BPSL0281) were amplified via Phusion® 328 polymerase (New England BioLabs) and cloned into a pET30a( Article Title: Optimization of Multivalent Gold Nanoparticle Vaccines Eliciting Humoral and Cellular Immunity in an In Vivo Model of Enterohemorrhagic Escherichia coli O157:H7 Colonization Article Snippet: EHEC (EDL933) genomic DNA was isolated via a Qiagen DNeasy blood and tissue kit according to the manufacturer’s directions. .. Sequences encoding LomW (GI:12514345), EscC (GI:12518466), LpfA1 (GI:12518278), and LpfA2 (GI:12518581) were amplified via Phusion polymerase (New England BioLabs) and cloned into a pET30a( Clone Assay:Article Title: Development of a Gold Nanoparticle Vaccine against Enterohemorrhagic Escherichia coli O157:H7 Article Snippet: EHEC (EDL933) DNA was isolated via the use of a Qiagen DNeasy blood and tissue kit, following the manufacturer’s directions. .. Sequences encoding LomW (GenBank protein accession no. 12514345) and EscC (GenBank protein accession no. 12518466) were amplified via the use of Phusion polymerase (New England BioLabs) and cloned into a Article Title: Development of a Gold Nanoparticle Vaccine against Enterohemorrhagic Escherichia coli O157:H7 Article Snippet: EHEC (EDL933) DNA was isolated via the use of a Qiagen DNeasy blood and tissue kit, following the manufacturer’s directions. .. Sequences encoding LomW (GenBank protein accession no. 12514345 ) and EscC (GenBank protein accession no. 12518466 ) were amplified via the use of Phusion polymerase (New England BioLabs) and cloned into a pET30a( Article Title: Use of Reverse Vaccinology in the Design and Construction of Nanoglycoconjugate Vaccines against Burkholderia pseudomallei Article Snippet: 321 322 Cloning and Expression of Burkholderia Antigens 323 B. mallei ATCC 23344 DNA was isolated via QIAGEN® DNeasy® Blood and Tissue Kit, 324 according to manufacturer’s directions. .. Sequences encoding OmpW (BMA2010/ BPSL2704), 325 Porin OpcP1 (BMAA1122/BPSS0708), Hemagglutinin (BMAA1324/BPSS0908), Hcp1 326 (BMAA0742/BPSS1498), FlgD (BMA3327/BPSL0272), OpcP porin (BMAA1353/ BPSS0879), 327 porin (BMAA0599/BPSS0757) and FlgL (BMA3336/BPSL0281) were amplified via Phusion® 328 polymerase (New England BioLabs) and cloned into a pET30a( Article Title: Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producing Citrobacter rodentium mouse model. Article Snippet: EHEC (EDL933) DNA was isolated with a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s directions. .. To improve recombinant protein solubility, the N-termi nus signal sequences of EscC (GenBank protein accession no. 12518466) and LomW (GenBank protein accession no. 12514345) were predicted using the SignalP 6.0 server, and the DNA segments without the predicted signal sequences were cloned into a pET30a( Article Title: Optimization of Multivalent Gold Nanoparticle Vaccines Eliciting Humoral and Cellular Immunity in an In Vivo Model of Enterohemorrhagic Escherichia coli O157:H7 Colonization Article Snippet: EHEC (EDL933) genomic DNA was isolated via a Qiagen DNeasy blood and tissue kit according to the manufacturer’s directions. .. Sequences encoding LomW (GI:12514345), EscC (GI:12518466), LpfA1 (GI:12518278), and LpfA2 (GI:12518581) were amplified via Phusion polymerase (New England BioLabs) and cloned into a pET30a( Article Title: Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producing Citrobacter rodentium mouse model Article Snippet: EHEC (EDL933) DNA was isolated with a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s directions. .. To improve recombinant protein solubility, the N-terminus signal sequences of EscC (GenBank protein accession no. 12518466) and LomW (GenBank protein accession no. 12514345) were predicted using the SignalP 6.0 server, and the DNA segments without the predicted signal sequences were cloned into a pET30a( Expressing:Article Title: Development of a Gold Nanoparticle Vaccine against Enterohemorrhagic Escherichia coli O157:H7 Article Snippet: EHEC (EDL933) DNA was isolated via the use of a Qiagen DNeasy blood and tissue kit, following the manufacturer’s directions. .. Sequences encoding LomW (GenBank protein accession no. 12514345) and EscC (GenBank protein accession no. 12518466) were amplified via the use of Phusion polymerase (New England BioLabs) and cloned into a Article Title: Development of a Gold Nanoparticle Vaccine against Enterohemorrhagic Escherichia coli O157:H7 Article Snippet: EHEC (EDL933) DNA was isolated via the use of a Qiagen DNeasy blood and tissue kit, following the manufacturer’s directions. .. Sequences encoding LomW (GenBank protein accession no. 12514345 ) and EscC (GenBank protein accession no. 12518466 ) were amplified via the use of Phusion polymerase (New England BioLabs) and cloned into a pET30a( Article Title: Use of Reverse Vaccinology in the Design and Construction of Nanoglycoconjugate Vaccines against Burkholderia pseudomallei Article Snippet: 321 322 Cloning and Expression of Burkholderia Antigens 323 B. mallei ATCC 23344 DNA was isolated via QIAGEN® DNeasy® Blood and Tissue Kit, 324 according to manufacturer’s directions. .. Sequences encoding OmpW (BMA2010/ BPSL2704), 325 Porin OpcP1 (BMAA1122/BPSS0708), Hemagglutinin (BMAA1324/BPSS0908), Hcp1 326 (BMAA0742/BPSS1498), FlgD (BMA3327/BPSL0272), OpcP porin (BMAA1353/ BPSS0879), 327 porin (BMAA0599/BPSS0757) and FlgL (BMA3336/BPSL0281) were amplified via Phusion® 328 polymerase (New England BioLabs) and cloned into a pET30a( Article Title: Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producing Citrobacter rodentium mouse model. Article Snippet: EHEC (EDL933) DNA was isolated with a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s directions. .. To improve recombinant protein solubility, the N-termi nus signal sequences of EscC (GenBank protein accession no. 12518466) and LomW (GenBank protein accession no. 12514345) were predicted using the SignalP 6.0 server, and the DNA segments without the predicted signal sequences were cloned into a pET30a( Article Title: Further Evaluation of Enterohemorrhagic Escherichia coli Gold Nanoparticle Vaccines Utilizing Citrobacter rodentium as the Model Organism Article Snippet: Briefly, DNA from EHEC strain EDL933 was extracted with a DNeasy Blood and Tissue kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions. .. To enhance solubility of recombinant EscC (GenBank protein accession no. 12518466), the N-terminal signal sequence of the protein was predicted using the SignalP 6.0 program, and the DNA segment without the predicted signal sequence was inserted in-frame with an ^X His-tag at the C-terminus into a pET30a( Article Title: Crystal Structure of H227A Mutant of Arginine Kinase in Daphnia magna Suggests the Importance of Its Stability Article Snippet: These PCR products, containing mutated gene, was extracted by QIAquick Gel Extraction Kit (Hilden, Germany, QIAGEN) and utilized to second PCR step as primer with primer Dm AK-NdeI-F (Forward) and Dm AK-NotI-R, which didn’t use at first PCR. .. The mutated genes, after second PCR, were inserted into the Article Title: Optimization of Multivalent Gold Nanoparticle Vaccines Eliciting Humoral and Cellular Immunity in an In Vivo Model of Enterohemorrhagic Escherichia coli O157:H7 Colonization Article Snippet: EHEC (EDL933) genomic DNA was isolated via a Qiagen DNeasy blood and tissue kit according to the manufacturer’s directions. .. Sequences encoding LomW (GI:12514345), EscC (GI:12518466), LpfA1 (GI:12518278), and LpfA2 (GI:12518581) were amplified via Phusion polymerase (New England BioLabs) and cloned into a pET30a( Article Title: Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producing Citrobacter rodentium mouse model Article Snippet: EHEC (EDL933) DNA was isolated with a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s directions. .. To improve recombinant protein solubility, the N-terminus signal sequences of EscC (GenBank protein accession no. 12518466) and LomW (GenBank protein accession no. 12514345) were predicted using the SignalP 6.0 server, and the DNA segments without the predicted signal sequences were cloned into a pET30a( Recombinant:Article Title: Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producing Citrobacter rodentium mouse model. Article Snippet: EHEC (EDL933) DNA was isolated with a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s directions. .. To improve recombinant protein solubility, the N-termi nus signal sequences of EscC (GenBank protein accession no. 12518466) and LomW (GenBank protein accession no. 12514345) were predicted using the SignalP 6.0 server, and the DNA segments without the predicted signal sequences were cloned into a pET30a( Article Title: Further Evaluation of Enterohemorrhagic Escherichia coli Gold Nanoparticle Vaccines Utilizing Citrobacter rodentium as the Model Organism Article Snippet: Briefly, DNA from EHEC strain EDL933 was extracted with a DNeasy Blood and Tissue kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions. .. To enhance solubility of recombinant EscC (GenBank protein accession no. 12518466), the N-terminal signal sequence of the protein was predicted using the SignalP 6.0 program, and the DNA segment without the predicted signal sequence was inserted in-frame with an ^X His-tag at the C-terminus into a pET30a( Article Title: Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producing Citrobacter rodentium mouse model Article Snippet: EHEC (EDL933) DNA was isolated with a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s directions. .. To improve recombinant protein solubility, the N-terminus signal sequences of EscC (GenBank protein accession no. 12518466) and LomW (GenBank protein accession no. 12514345) were predicted using the SignalP 6.0 server, and the DNA segments without the predicted signal sequences were cloned into a pET30a( Solubility:Article Title: Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producing Citrobacter rodentium mouse model. Article Snippet: EHEC (EDL933) DNA was isolated with a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s directions. .. To improve recombinant protein solubility, the N-termi nus signal sequences of EscC (GenBank protein accession no. 12518466) and LomW (GenBank protein accession no. 12514345) were predicted using the SignalP 6.0 server, and the DNA segments without the predicted signal sequences were cloned into a pET30a( Article Title: Further Evaluation of Enterohemorrhagic Escherichia coli Gold Nanoparticle Vaccines Utilizing Citrobacter rodentium as the Model Organism Article Snippet: Briefly, DNA from EHEC strain EDL933 was extracted with a DNeasy Blood and Tissue kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions. .. To enhance solubility of recombinant EscC (GenBank protein accession no. 12518466), the N-terminal signal sequence of the protein was predicted using the SignalP 6.0 program, and the DNA segment without the predicted signal sequence was inserted in-frame with an ^X His-tag at the C-terminus into a pET30a( Article Title: Efficacy of EHEC gold nanoparticle vaccines evaluated with the Shiga toxin-producing Citrobacter rodentium mouse model Article Snippet: EHEC (EDL933) DNA was isolated with a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s directions. .. To improve recombinant protein solubility, the N-terminus signal sequences of EscC (GenBank protein accession no. 12518466) and LomW (GenBank protein accession no. 12514345) were predicted using the SignalP 6.0 server, and the DNA segments without the predicted signal sequences were cloned into a pET30a( Sequencing:Article Title: Further Evaluation of Enterohemorrhagic Escherichia coli Gold Nanoparticle Vaccines Utilizing Citrobacter rodentium as the Model Organism Article Snippet: Briefly, DNA from EHEC strain EDL933 was extracted with a DNeasy Blood and Tissue kit (Qiagen, Germantown, MD, USA), according to the manufacturer’s instructions. .. To enhance solubility of recombinant EscC (GenBank protein accession no. 12518466), the N-terminal signal sequence of the protein was predicted using the SignalP 6.0 program, and the DNA segment without the predicted signal sequence was inserted in-frame with an ^X His-tag at the C-terminus into a pET30a( Polymerase Chain Reaction:Article Title: Crystal Structure of H227A Mutant of Arginine Kinase in Daphnia magna Suggests the Importance of Its Stability Article Snippet: These PCR products, containing mutated gene, was extracted by QIAquick Gel Extraction Kit (Hilden, Germany, QIAGEN) and utilized to second PCR step as primer with primer Dm AK-NdeI-F (Forward) and Dm AK-NotI-R, which didn’t use at first PCR. .. The mutated genes, after second PCR, were inserted into the |